The 22 Northern Premier League Premier Division teams join the competition at this stage, to join the remaining Step 4 clubs from the NPL that have navigated the preliminary and extra preliminary rounds.
Games will take place on Saturday September 2. Winners will receive £2,250 in prize money, with losers receiving £750.
Charnock Richard v Runcorn Linnets
Ashton United v Dunston
Prescot Cables v North Ferriby
Clitheroe v Hyde United
Guisborough Town v Atherton Collieries
Consett v Bradford (Park Avenue)
Ossett United v Marske United
Warrington Rylands v Stockton Town
Irlam or Goole v Whitby Town
Bamber Bridge v Mossley
Whickham or Whitley Bay v Macclesfield
Brighouse Town v Albion Sports
Guiseley v Marine
Workington v Prestwich Heys
FC United v Whitchurch Alport or Barnoldswick Town
Widnes v Lower Breck or Emley
Morpeth Town v Radcliffe
Lancaster City v Witton Albion
Nantwich Town v Shifnal Town
Anstey Nomads v Hanley Town or Stourport Swifts
Redditch United v Boldmere St Michaels
Cleethorpes Town v Hinckley LR
Lincoln United v Kidsgrove Athletic
Sporting Khalsa v Darlaston Town
Stratford Town v Spalding United or Shepshed Dynamo
Rugby Town v Barwell
Worksop Town v Sheffield
Stafford Rangers v Atherstone Town
Leamington v Coleshill Town
Alvechurch v Basford United
Mickleover v Ilkeston Town
Nuneaton Borough v Carlton Town
Harborough Town v Stamford
Gainsborough Trinity v Matlock Town
Leek Town v Grimsby Borough
Leighton Town v Cambridge City
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