The stand out game in the Premier Division sees Scarborough Athletic host South Shields in a real clash of the titans which could have a big impact on the play-off places.
Leaders Nantwich Town are at Stafford Rangers, while Warrington Town also have a local derby away at Witton Albion.
Two play-off chasers meet at Silverlands as Buxton host Matlock Town, where a win could lift either in to the play-offs.
Gladiators boss Dave Frecklington said: "It's our first derby and we want to win it, the lads will be fired up for it and will want to give a really good account of themselves.
“Having lost in the way we did at South Shields, the lads have responded magnificently to win three on the trot in the league and make the semi finals of the Derbyshire Senior Cup. Buxton are a good side and we know the threat they possess, but we'll be going there looking for the victory."
Out of form Hednesford Town face a banana skin trip to bottom placed Hednesford Town and Farsley Celtic welcome Whitby Town amongst the other games.
In the East Division, leaders Morpeth Town face a tricky trip to Marske United knowing a slip up could enable Brighouse and Tadcaster, at home to Pickering and Spalding respectively, to catch them on points.
Cleethorpes Town continue their climb up the table with a home win over Lincoln United, while Pontefract Collieries host Ossett United in another highlight fixture.
In the west, new leaders Radcliffe face a stern test of their seven game winning run as they visit Ramsbottom United. Runcorn Linnets have a derby match against Widnes as they look to bounce back from their surprise home defeat to Droylsden at the weekend.
Two play-off chasers meet at Shawe View too, as Trafford host Atherton Collieries.
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