Games will take place on Saturday September 18, with winning clubs receiving £3,375 in prize money and losers receiving £1,125.
Five first qualifying round replays involving Northern Premier League sides will take place on Tuesday evening.
York City v Hebburn Town
Marine v Warrington Town
Atherton Collieries or Bridlington Town v Witton Albion
Squires Gate v Pontefract Collieries
Blyth Spartans v Bootle or FC United
Colne v Guiseley
Curzon Ashton v Stockton Town
City of Liverpool v Farsley Celtic
Morpeth Town v Lancaster City
Mossley v Radcliffe
Prescot Cables v Whitby Town
Runcorn Linnets v Bamber Bridge
Marske United v South Shields
Belper Town v Tamworth
Bromsgrove Sporting v Worksop Town
Sporting Khalsa v Kidderminster Harriers
Sherwood Colliery or Buxton v Rushall Olympic or Stafford Rangers
Boston United v Corby Town
Nantwich Town v Banbury United
AFC Telford United v Stamford
Spalding United v Kettering Town
Hinkley LR or Hanley Town v Chasetown
Bedworth United v Long Eaton United
Halesowen Town v Handsworth
Hednesford Town or Cleethorpes Town v Alfreton Town
Mickleover v Basford United
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