There are 37 Northern Premier League sides remaining in the competition, with 30 of these going head-to-head for a spot in the First Round Proper.
Games will take place on Saturday 7 October. Winners will receive £2,450, with losers receiving £625.
Avro v Warrington Rylands
Bootle v Bamber Bridge
North Ferriby v Atherton Collieries
Bradford (Park Avenue) v Nantwich Town
Lancaster City v City of Liverpool
Hanley Town v Macclesfield
Morpeth Town v FC United of Manchester
Ashton United v Brighouse Town
Workington v Hyde United
Radcliffe v Whitby Town
Gainsborough Trinity v Guiseley
Worksop Town v Stocksbridge Park Steels
Marske United v Marine
Quorn v AFC Telford United
Stratford Town v Basford United
Anstey Nomads v Alvechurch
Boldmere St Michaels v Leighton Town
Stafford Rangers v Sporting Khalsa
Matlock Town v Bedford Town
Ilkeston Town v Walsall Wood
Nuneaton Borough v Carlton Town
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